Product Detail | |
---|---|
Description | The Human TCR (with molecular barcodes) is driven by a robust chemical method that provides unparalleled sensitivity and reproducibility. The kit utilizes the SMART (5' end of RNA template conversion mechanism) full-length cDNA synthesis technology, pairing NGS with the 5'-RACE method to capture the intact V(D)J variable region of the human TRA and TRB genes. Libraries can be generated to obtain information on the diversity of alpha and beta strands. |
Nucleic Acid Type | RNA |
Species Category | Human |
Specialized Sample Types | The kit is designed to handle a range of RNA input volumes (RIN ≥ 8; depending on sample type) and has been shown to yield high-quality total RNA from peripheral blood leukocytes down to 10 ng to 1 µg, total RNA from whole blood from 20 ng to 200 ng, total RNA from T cells from 1 ng to 100 ng, or from 1,000 to 10,000 purified whole T cells sequencing libraries. |
Shipping Condition | Dry ice (-70 °C) |
Storage | Store at -20 °C. (Store Control RNA at -70 °C.) |