Mouse CD4+CD25+ Regulatory T Cell Isolation Kit
Catalog No: IMCE-01052
Size:
1 kit;5 kits;10 kits
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| Product Detail |
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| Species Category |
Mouse |
| Description |
The Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II is designed to isolate CD4+CD25+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection and positive selection. CD4+ T cells are first enriched by negative selection using the Mouse CD4+ T Cell Isolation Cocktail. CD25+ cells were then positively selected from the pre-enriched cells using the Mouse CD25 Regulatory T Cell Positive Selection Cocktail. |
| Note |
For Processing 10^9 cells |
| Shipping Condition |
Room Temperature |
| Storage |
Store at 2 - 8°C. Do not freeze. (Normal Rat Serum: -20 °C) |
| Cell Purity |
Up to 93% purity |
| Component |
Mouse CD4+ T Cell Isolation Cocktail - A combination of monoclonal antibodies in PBS and 0.1% BSA.
Mouse CD25 Regulatory T Cell Positive Selection Cocktail - A combination of monoclonal antibodies in PBS, 0.1% BSA, and < 0.1% sodium azide.
PE Selection Cocktail - A combination of monoclonal antibodies in PBS.
Magnetic Particle Suspension - A suspension of magnetic particles in PBS.
Mouse FcR Blocker - A monoclonal antibody in PBS, 0.1% BSA, and < 0.1% sodium azide.
Normal Rat Serum - Mycoplasma-free normal rat serum. |
| Shelf Life |
Stable until expiry data (EXP) on label. |
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