Mouse CD4+CD62L+ T Cell Isolation Kit
Catalog No: IMCE-01053
Size:
1 kit;5 kits;10 kits
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| Product Detail |
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| Species Category |
Mouse |
| Description |
Highly purified naïve CD4+ T cells are isolated from single-cell suspensions of spleen cells or other tissues by a simple two-step method. First, mouse naïve CD4+ T cells were enriched by negative selection using a mouse naïve CD4+ T cell pre enrichment cocktail containing biotinylated antibodies against non-naive CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD45R, CD49b, TCRγ/δ, TER119). The PE selection cocktail was then used to select CD4+CD62L+ cells pre-labeled with CD62L PE. The desired cells were labeled with antibodies and magnetic particles. The cells are separated column-free using a magnet. |
| Note |
For Processing 10^9 cells |
| Shipping Condition |
Room Temperature |
| Storage |
Store at 2 - 8°C. Do not freeze. (Normal Rat Serum: -20 °C) |
| Cell Purity |
Up to 97% purity |
| Component |
Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail - A combination of monoclonal antibodies in PBS and 0.1% BSA.
PE Selection Cocktail - A combination of monoclonal antibodies in PBS.
Magnetic Particle Suspension
Normal Rat Serum - Mycoplasma-free normal rat serum. |
| Shelf Life |
Stable until expiry data (EXP) on label. |
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