B Cell Isolation Service

Understanding the antibody repertoires and the fate of B cells is essential for defining the underlying mechanisms and drug development for a wide range of diseases, particularly oncological and immunological diseases, including autoimmune and allergic diseases and transplant rejection. As an expert in immunomics, CD Genomics provides professional B cell isolation services to advance your immunomics-related projects.

Introduction to B Cells

In mammals, B cells, also known as B lymphocytes, are derived from the pluripotent stem cells of the bone marrow. When stimulated by antigens, these lymphocytes proliferate into plasma cells, which synthesize antibodies and function as humoral immunity. The differentiation process of mammalian B cells can be divided into five main stages: pre-B cells, immature B cells, mature B cells, activated B cells, and plasma cells.

Isolation Methods for B Cells

The isolation of B cells, the main carrier of humoral immunity, is essential for immunomics studies and there are two main types of B cell isolation, which are immunomagnetic bead-based isolation methods and flow sorting methods.

• Immunomagnetic Bead Method
  The immunomagnetic bead method of cell separation is based on the ability of cell surface antigens to bind to specific monoclonal antibodies attached to magnetic beads. In an applied magnetic field, cells attached to the beads by antibodies are adsorbed and retained in the magnetic field. Cells that cannot bind to the specific monoclonal antibody attached to the magnetic beads do not remain in the magnetic field, thus allowing the cells to be separated. Depending on the relationship between the cells bound to the magnetic beads and the cells to be obtained, the immunomagnetic bead method can be divided into positive and negative selection methods. The positive selection method means that the cells bound to the beads are the cells to be obtained, while the negative selection method means that the beads bind the unwanted cells and the cells free from the supernatant are the desired cells.
• Flow Sorting Methods
  Flow sorting is one of the more widespread B cell isolation techniques available and is based on the specificity of the antigen-antibody reaction. Antigen-specific B cells are screened by multicolor flow analysis and sorting using fluorescein-labeled antibodies to B cell surface molecules. Flow sorting allows individual antigen-specific B cells to be sorted directly into a single well of a cell culture plate for subsequent experiments.

Our Services

The isolation of B cells facilitates the subsequent more precise phenotypic, functional, and molecular definition of the B cell and plasma cell populations and the comprehensive analysis of the antigenic reactivity of the antibodies they express.

Multiple Types of Samples

Based on many years of experience in immunomics research, CD Genomics offers a professional and reliable B cell isolation service for biomaterials from human, non-human primates, mice, and rats. Our B cell isolation services can target a wide range of sample types, including but not limited to the following.

Highly Customizable Specific B Cell Isolation

Our B cell isolation services is highly customizable. Different types of B cells are isolated depending on the needs of the client and the different projects, such as naive B cells, memory B cells, and HLA Crossmatch B Cells.

Workflow of B Cell Isolation

Why Choose Us

	High Purity: the purity of the obtained cells reaches (90%-99%).
	Great Yield: the obtained rate is up to 90%.
	Low Impact on Cell Viability: the rate of viable cells is greater than 95%.

CD Genomics is experienced in the field of immunomics. Over the years we have provided professional and high-quality B cell isolation services to many institutions and research groups. Please contact us for more information.

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