The immune system is composed of a variety of cells that protect the body in a coordinated manner from a multitude of different pathogens. The development of single-cell analysis techniques promises to facilitate the detection of less common cell types and transient or intermediate cell states. CD Genomics offers several types of single-cell histology analysis services to help clients gain new insights into the diversity of the immune system.
Multiple omics studies of single cells enable the definition of distinct human immune cell types in multiple tissue and disease contexts. These techniques improve the identification of heterogeneous cell types and states in the human immune system.
For example, based on the single-cell omics analyses of more than 780,000 peripheral blood mononuclear cells from 130 COVID-19 patients of varying severity, it is found that clonally expanded CD8+ T cells and increased ratio of CD8+ effector T cells to effector memory T cells are identified as features of severe disease, while the increasing of circulating follicular helper T cells is accompanied by mild disease.
Fig.1 Bar plot of the proportion of cell
types. (Stephenson, E., et al., 2021)
Single-cell technologies enable the analysis of large numbers of cells, making them a powerful tool for describing rare cell types and refining our understanding of different cellular states. In addition, single-cell omics can help unravel the mechanisms involved in disease pathogenesis and outcome.
Hence, CD Genomics offers a variety of single-cell approaches, including genomics, transcriptomics, epigenomics, proteomics, and poly-genomics, to obtain valuable data on the dynamics of the immune system.
Single-cell DNA sequencing (scDNA-seq) facilitates the revelation of intercellular variation and heterogeneity at the genomic level. Our scDNA-seq services allow the study of single nucleotide variation, copy number variation, and microsatellite variation.
Cell populations with homogeneous cell surface markers hide intercellular differences that have a considerable impact on cell function. This heterogeneity can be addressed by single-cell RNA sequencing (scRNA-seq). Not only can we focus on messenger RNAs (mRNAs), but also help our customers analyze microRNAs and other regulatory RNAs.
Epigenetics is a complex regulatory network that regulates chromatin structure and genome function through chemical modifications of DNA and histones. We offer services to analyze methylation patterns, histone modifications, and chromatin states for single cells.
Proteomic analysis at the single-cell level can provide additional information about cellular state and function. CD Genomics offers analysis services for single-cell proteomics based on fluorescence-activated cell sorting and single-cell mass spectrometry (CyTOF) with moderate throughput (about 40 to 50 markers).
CD Genomics offers genome and transcriptome sequencing (G&T-seq), which interrogates both DNA and mRNA of individual cells to dissect the effects of genomic variation on gene expression. We also offer single-cell analysis of the epigenome and transcriptome to help our clients study gene regulatory pathways.
The implementation of computational methods is critical to extracting biological information from the data. CD Genomics provides professional data analysis services to help clients handle high-dimensional data, integrate data from different experimental groups, and analyze multiple types of data.
CD Genomics has a specialized technology platform that provides a variety of single-cell histology analysis services targeting immune cells to help our clients understand the complex immune process. Please contact us for more information.
