Transcriptomics is the study of gene expression from the RNA level. The transcriptome, the sum of all RNAs that can be transcribed from a living cell, is an important tool for studying cellular phenotype and function. CD Genomics provides professional transcriptomics analysis services in immunomics to help our customers solve problems.
Transcriptome analysis constitutes a genomic approach as it consists of measuring transcript abundance in a sample on a genome-wide scale and relies on the detection or sequencing of oligonucleotide sequences present in the sample. The use of systematic methods is changing the landscape of biomedical research by enabling comprehensive molecular analysis of cells and tissues obtained from human subjects in health and disease.
Fig.1 Diagram of the transcriptome.
Transcriptomics has been widely used to study immune processes involved in health maintenance and disease pathogenesis in human research, such as infectious diseases, autoimmunity, cancer, neurological diseases, cardiovascular diseases, nutritional deficiencies, pregnancy, and other diseases.
Quantitative gene expression measurements using complementary oligonucleotide probes. Targeted transcriptome approaches that investigate defined genomes reduce the complexity of the analysis, the depth of sequencing required, and thus the cost considerably, but also limit the possible observations to predefined genomes.
NGS-based analysis of total RNA or mRNA with full transcript coverage. Genome-wide and full-length assessment of RNA molecules allow transcriptome analysis at the transcript heterodimer level. High sensitivity and high resolution are the two greatest advantages of this method.
Human samples include but are not limited to cancerous tissues such as the liver, lung, and stomach, and non-cancerous tissues such as plaque, blood, blood vessels, and skin.
Mouse samples include but are not limited to cancerous tissues such as the small intestine, rectum, melanoma, and non-cancerous tissues such as meninges, heart, and bone marrow.
CD Genomics has been focused on conducting transcriptomic studies in immunomics for many years. Through collaboration and communication with our clients, we have provided solutions for several studies that have advanced the mechanistic study of multiple immune-related diseases. Please contact us for more information.
