Mucosal Immune System Protein Profiling by CyTOF

High-dimensional mass spectrometry (Cytometry by time-of-flight, CyTOF) offers the possibility to analyze many cellular markers simultaneously, providing an opportunity to analyze the mucosal immune system at an unprecedented resolution. CD Genomics offers professional mucosal immune system protein profiling by CyTOF to interrogate all major lineage immune subpopulations simultaneously on a single-cell basis at a system-wide level.

Introduction to the Mucosal Immune System

The mucosal surface is continuously exposed to the external environment and therefore represents the largest lymphoid organ in the body. Within the mucosal immune system, gut-associated lymphoid tissues (GALTs), including Peyer's patches and isolated lymphoid follicles, play an important role in the induction of antigen-specific immune responses in the gut. GALTs have unique organogenesis characteristics and interact with dendritic cells and T-cell networks to both induce and regulate IgA responses and oral tolerance.

Applications of CyTOF in Mucosal Immune System Protein Profiling

Mass spectrometry (CyTOF) is an advanced technique for the multidimensional analysis of protein expression that facilitates multiparametric immunophenotyping of individual cells. Multiparametric phenotypic measurements at single-cell resolution facilitate high-dimensional analysis and visualization of relationships between successive developing lymphocyte subpopulations, rather than as static features of the entire cell pool.

• CyTOF Reveals Innate Lymphoid Cell Differentiation in the Fetal Intestine
  Innate lymphocytes (ILCs) are abundant in mucosal tissues and are involved in tissue homeostasis and barrier function. To exemplify the determination of their differentiation pathways, ILCs in the human fetal gut are analyzed using mass spectrometry and 34 distinct populations are distinguished. The results identify a smooth phenotypic transition from cells within the Lin^-CD7⁺CD127^-CD45RO⁺CD56⁺CD8a^- cluster to CD45RA⁺ NK cells and CD127⁺RORγt⁺ ILC3-like cells, revealing potential differentiation trajectories.

Fig.1 Schematic map of CyTOF for identification of innate lymph-like cell differentiation pathways. (Li, N., et al., 2018)

• Mucosal Immune System Protein Profiling by CyTOF to Identify Inflammatory Intestinal Disease State
  The inflammatory intestinal disease is characterized by an abnormal immune response and affects different locations in the gastrointestinal tract. A system-wide view of the human mucosal immune system in health and disease is performed using high-dimensional CyTOF, which distinguishes 142 immune subpopulations and detects an ILC3-like subpopulation that is present only in rectal biopsies from patients with Crohn's disease (CD) in remission.

Our Services

CyTOF is a valuable tool in the field of immunology because it allows for high-resolution profiling of the composition of the immune system at the cellular level. Advances in CyTOF technology offer the possibility of simultaneous measurement of multiple cellular protein markers (>40).

• Phenotype Assay Services
  CD Genomics provides efficient and high-quality mucosal immune system protein profiling by CyTOF that can be used to identify typical or novel cell populations, including the characterization of cell population heterogeneity for specific cancers, the specification of characteristic cell populations in the analysis of specific diseases, and the monitoring of the immune system response to various infections. Our experts provide the best panel design for your project to help you overcome the challenges of your project.
• Data Analysis Services
  CyTOF provides the ability to analyze dozens of single-cell markers simultaneously on complex cell samples, resulting in highly complex datasets. Traditional methods of flow cytometry data analysis are not suitable for such datasets, suffer from individual user bias, and require prior knowledge of the cell type of interest. CD Genomics offers data analysis services based on SPADE, t-SNE, and ACCENSE to visualize and delineate phenotypically distinct subsets.

Workflow of Mucosal Immune System Protein Profiling by CyTOF

Why Choose Us

CD Genomics pioneers in single-cell analysis targeting immune cells. Our scientists are experts in CyTOF and immunology and have been performing single-cell assay experiments targeting the immune system for many years. We ensure that you are properly advised on assay design and get the best panel setup for your project. Please contact us for more information.

Reference

1. Li, N., van Unen, V., Höllt, T., Thompson, A., van Bergen, J., Pezzotti, N., ... & Koning, F. (2018). Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine. Journal of Experimental Medicine, 215(5), 1383-1396.

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