Peripheral Blood Mononuclear Cell Isolation Service

Peripheral Blood Mononuclear Cell (PBMC) is a key component of the immune system and are therefore used as the most fundamental experimental material in immunology, immunomics, infectious diseases, malignancies, vaccine development, transplantation therapy, personalized medicine, and toxicology. CD Genomics provides professional PBMC isolation services to clients worldwide to advance immunomics projects.

What Is PBMC?

PBMC refers to cells in the peripheral blood that have a single nucleus, including lymphocytes, monocytes, dendritic cells (DC), and a small number of other cells. The proportion of different subtypes in PBMC varies from person to person, but on average, lymphocytes make up the majority of PBMC (70%-90%). In addition, PBMC contains a small proportion of dendritic cells (1-2%), which form an important bridge between the innate and adaptive immune systems.

Research Implications of PBMC

Peripheral blood is the place of exposure to chemicals making PBMC susceptible to the effects of drugs and chemicals. This is why the availability of peripheral blood PBMC is so important to researchers studying the toxicity of new drugs or compounds. PBMC has been used extensively to assess several aspects of immune regulation.

PBMC Isolation by Density Gradient Centrifugation

PBMC is isolated from peripheral blood by density gradient centrifugation. Density gradient centrifugation, more widely known as isopycnic centrifugation, is a technique in which macromolecules move through a density gradient until they find the same density as their own. The most common layering solutions used in PBMC isolation include Ficoll and Percoll.

• PBMC Isolation by Ficoll Gradient
  The Ficoll density gradient centrifugation method is the most used method for the isolation and purification of PBMC. This method mainly uses the difference between the specific gravity of various blood cells and individual nucleated cells for separation. Ficoll is a neutral polymorph of sucrose with an average molecular weight of 400,000. When the density is 1.2g/mL, it does not exceed the normal physiological osmotic pressure and does not cross biological membranes.
• PBMC Isolation by Percoll Gradient
  Percoll is a suspension of silica particles treated with polyvinyl pyrrolidone (PVP), which is non-toxic and non-irritating to the cells. The silica particles in Percoll suspensions are of different sizes and are centrifuged at high speed to create a continuous density gradient to separate and purify cells of different specific gravity. Percoll suspension is a promising method for purifying lymphocytes and monocytes, with a purity of up to 98% for lymphocytes and 78% for monocytes.

Our Services

PBMCs are a key component of the immune system. Similarly, the PBMC transcriptional profile is an important component of immunohistology. The study of PBMC is of great importance to facilitate disease modeling, drug toxicity screening, drug discovery, and personalized medicine with cell replacement therapy.

CD Genomics has been dedicated to immunomics research for many years and has accumulated a wealth of experience. For your special projects, CD Genomics offers PBMC isolation from the peripheral blood of human, non-human primates, mice, and rats to generate reliable experimental materials for your immunomics projects.

Workflow of Peripheral Blood Mononuclear Cell Isolation

Why Choose Us

	High Purity: the purity of the obtained cells reaches (90%-99%).
	Great Yield: the obtained rate is up to 90%.
	Low Impact on Cell Viability: the rate of viable cells is greater than 95%.

CD Genomics is a pioneer in immunomics research. We are committed to advancing the convergence of omics and immunology to provide premium solutions for your projects. Please contact us for more information.

Reference

1. Liu, Q., Du, J., Fan, J., Li, W., Guo, W., Feng, H., & Lin, J. (2019). Generation and characterization of induced pluripotent stem cells from mononuclear cells in schizophrenic patients. Cell Journal (Yakhteh), 21(2), 161.

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