Serum Proteomics Analysis Service

Serum is a complex body fluid containing a variety of proteins in at least nine orders of magnitude. Serum is mainly used for blood biochemical tests and tumor marker detection. CD Genomics provides high-quality serum proteomics services to facilitate the discovery of novel protein biomarkers for various diseases.

Introduction to Serum Proteomics

Serum is derived from plasma with clotting factors removed and contains 60-80 mg of protein/mL in addition to various small molecules including salts, lipids, amino acids, and sugars. The major protein components of serum include albumin, immunoglobulins, transferrin, leptin, and lipoproteins. In addition to these major components, the serum contains many other proteins that are synthesized and secreted, shed, or lost from cells and tissues throughout the body. It is estimated that up to 10,000 proteins may typically be present in serum, most of which will be present in very low relative abundance.

Serum Proteomics for Disease Marker Discovery

One of the driving forces of proteomics is the discovery of biomarkers, that is, proteins that change in concentration or state in relation to a specific biological process or disease. Determining relative or absolute concentration changes is the basis for the discovery of validated biomarkers. Serum proteomics facilitates the discovery of biomarkers for a wide range of diseases, particularly cancer biomarkers.

• Biomarker Discovery for Endometrial Cancer
  Endometrial cancer is the most common gynecologic cancer in developed countries and the second most common gynecologic cancer in the world. Despite its high incidence, there are no available screening protocols, no standard optimal therapy, and no optimal surveillance methods. A label-free quantitative method using LC-MS/MS was used to quantify differentially expressed proteins in endometrial cancer. Seventeen candidate proteins were identified specifically in patients with malignancies. Among these 17 proteins, FAM83D was the one with the greatest biomarker potential. Fig.1 Ingenuity pathway analysis analysis of 17 biomarkers of endometrial cancer. (Uyar, D. S., et al., 2021)
• Biomarker Discovery for Allergic and Nonallergic Asthma
  Asthma is a heterogeneous disease with different phenotypes, endotypes, and severity. Through MS analysis, 18 proteins with different abundance are identified, such as IGFALS, HSPG2, FCN2, and MASP1. Among them, IGFALS is a useful biomarker for moderate-severe allergic asthma. Functional/network studies of these proteins identified key processes in asthma pathogenesis, such as complement activation, extracellular matrix organization, platelet activation and degranulation, and post-translational protein phosphorylation.

Our Services

Blood constitutes an unlimited source of analytes that may become potential biomarkers. The enthusiasm for biomarker research illustrates the success and potential of the science of omics. Serum proteomics focuses on the biological expression changes of genes and is necessary to understand the mechanisms underlying physiological or pathological states.

CD Genomics uses a variety of mass spectrometry platforms to provide customers with reliable serum proteomics analysis services. Combined with professional bioinformatics analysis, including data processing, significance analysis, and pathway analysis, we offer our clients comprehensive serum proteomics analysis services.

Workflow of Serum Proteomics Analysis

Why Choose Us

CD Genomics is a pioneer in immunology research. We are dedicated to the analysis and study of serum and plasma proteomes, providing reliable data and a full range of analyses for your project. Please contact us for more information.

Reference

1. Uyar, D. S., Huang, Y. W., Chesnik, M. A., Doan, N. B., & Mirza, S. P. (2021). Comprehensive serum proteomic analysis in early endometrial cancer. Journal of proteomics, 234, 104099.

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