Epigenetic changes have been associated with aspects of immune cell development, activation, and differentiation. A genome-wide epigenetic landscape has been established for some immune cells, including tissue-resident macrophages and blood-derived cells, including T cells. CD Genomics offers epigenomics services for immune cells to advance our clients' insights into the general rules of epigenetic regulation in the immune process.
In terms of single-cell analysis, the transcriptome and protein levels of immune cells have been explored. However, the characteristics of chromatin accessibility, cis-regulatory elements, and trans-factors driving epigenetic cellular states that are critical for the transcriptional regulation of genes at critical stages of the immune process remain unclear.
Epigenetics has an important role in cells of the innate and adaptive immune system. The responsiveness to environmental cues and the reversibility of the epigenetic landscape allows for plasticity in the transcriptional programs of already differentiated cells. CD Genomics offers professional single-cell epigenomics services for immune cells to help our clients decipher the immune progress.
We offer bisulfite conversion-based methods being used for single-cell methylation sequencing, including whole genome bisulfite sequencing (WGBS), where the entire genome is analyzed, and reduced representation bisulfite sequencing (RRBS), in which regions with high CpG content (CpG islands) are enriched and sequenced.
We have a variety of standard antibodies that have been used for single-cell histone modification analysis, including H3K4me1, H3K4me3, H3K9me3, H3K27me3, H3K36me3, H3K27Ac, and CTCF. In addition, we can use monoclonal antibodies labeled against other histones, after performing titrations to determine the correct concentration for the experiment.
Single-cell ATAC-Seq (scATAC) relies on the transposase-mediated insertion of sequencing primers into open chromatin regions. Our scATAC-Seq service can examine chromatin accessibility in thousands of cells in parallel, allowing the examination of subpopulations of cells in heterogeneous populations.
Based on years of epigenomic research, CD Genomics provides clients with many types of single cell epigenomics analysis services to help them explore multiple immune processes. Please contact us for more information.
